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Figure 1 | Journal of Neuroinflammation

Figure 1

From: Minocycline prevents retinal inflammation and vascular permeability following ischemia-reperfusion injury

Figure 1

Minocycline (Mino) treatment significantly inhibited retinal vascular leakage and tight junction reorganization following ischemia-reperfusion (IR). Mino was delivered as twice-daily intraperitoneal (IP) injections, with two initial dosages of 45 mg/kg prior to ischemia and dosages of 22.5 mg/kg just prior to ischemia and every 12 h for the next 2 d during the reperfusion period as described in Materials and Methods. Non-treated (NT) animals received PBS vehicle injections. One eye of each rat was subjected to IR for 45 min or needle puncture only (Sham) and after 48 h of reperfusion retinas were assayed for (A) Evans blue dye leakage (n = 8 retinas per group), or (B and C) for vascular endothelium tight junction organization by immunohistochemistry of ZO-1 protein at endothelial cell borders (n = 4 retinas per group). Representative images from Sham and IR retinas are shown in B, with arrowheads indicating intact borders and arrows indicating regions of ZO-1 discontinuity. Distributions of endothelial cell border organization grading frequency are shown in C, with five representing fully continuous border staining and one representing complete loss of continuous border staining. *P ≤0.05, **P ≤0.01 and *** P ≤0.001 by Student’s t-test.

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