Figure 11

Hon inhibits IFNγ-induced activation of p-ERK1/2 in HAPI microglial cells. (A) Western blot analysis showing a representative experiment of Hon pretreatment on IFNγ to induce ERK1/2 phosphorylation in HAPI microglial cells. Cells were treated with Hon (1 to 10 μM) for 1 h followed by stimulation with IFNγ (10 ng/ml) for 4 h. (B &C) Results of protein band intensities are expressed as arbitrary units of phospho-ERK1/2 against total ERK1/2. Results are expressed as the mean ± SEM (n = 3) and significant difference from the respective IFNγ stimulated group was determined by one-way ANOVA followed by Dunnett’s tests, *P < 0.05; ** P < 0.01; *** P < 0.001.