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Figure 2 | Journal of Neuroinflammation

Figure 2

From: Magnolia polyphenols attenuate oxidative and inflammatory responses in neurons and microglial cells

Figure 2

Hon and Mag do not alter cell viability and inhibit NMDA-induced ROS production in primary rat cortical neurons. (A) Exposure of Hon and Mag (1 to 10 μM) to primary cortical neurons for 24 h did not alter neuronal viability as assayed by MTT and AlamarBlue. (B) Representative bright field and DHE fluorescence photomicrographs depicting ROS production in primary neurons after cells were treated with Hon or Mag (10 μM) for 30 min prior to stimulation with NMDA (100 μM) for 30 min. For ROS production, neurons were loaded with dihydroethidium (DHE, 10 μM) 30 min prior to image acquisition under different treatment conditions. Procedure for fluorescence determination is described in text. (C) Bar graph of average fluorescence depicting significant inhibition of NMDA-induced ROS production by Hon and Mag. Data are expressed as the mean ± SEM from three individual experiments and analyzed by two-way ANOVA with Bonferroni posttests. ** denote significant difference between control and NMDA (P < 0.001), ## indicate significant decrease in ROS production by Hon and Mag as compared to NMDA (P < 0.01).

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