Figure 3
From: Magnolia polyphenols attenuate oxidative and inflammatory responses in neurons and microglial cells
Hon and Mag inhibit LPS + IFNγ-induced NO production and iNOS expression in BV-2 microglial cells. Cells were treated with Hon or Mag (1 to 10 μM) for 1 h followed by stimulation with LPS (100 ng/ml) + IFNγ (10 ng/ml) for 16 h. (A) Culture media were collected for determination of NO using the Griess reaction protocol as described in text. (B) Representative Western blots of iNOS protein and β-actin. (C) Bar graphs representing iNOS/β-actin ratios. Results were expressed as the mean ± SEM (n = 3) and significant difference from the respective LPS + IFNγ stimulated group was determined by one-way ANOVA followed by Dunnett’s tests, ** P < 0.01; ***P < 0.001.