Figure 1

Cytokine levels induced by LPS or Aβ42 in primary tri-cultures. Cytokine (IL1-β, TNF-α, and IL-6) levels in culture medium (A, B, C, respectively) and in cell lysates (D, E, F, respectively) of tri-cultures pre-treated or not with 210 nM C16 and treated with 100 ng/mL LPS or with 20 μM Aβ42 in serum-free medium were analyzed by the 3-plex Luminex xMAP assay containing a mixture of specific beads for each cytokine as described in the Methods section. Cytokine levels in culture medium and cell lysates are expressed in pg/mL and pg/mg protein, respectively. Results are mean ± SEM for six experiments in duplicate. ^*** P <0.001 compared to control; ^## P <0.01, ^### P <0.001 compared to LPS; ^†† P <0.01, ^††† P <0.001 compared to C16 by one-way ANOVA with a Newman-Keuls multiple comparison test. LPS, lipopolysaccharide; NS, not significant.