Figure 10
Activation of mTOR signaling pathway in microglia. Representative immunoblots show the immunoreactivity of (A) Beclin-1, (B) mTOR, (C) PS2448-mTOR, p70S6K, PT389-p70S6K, and β-actin from cell lysates of primary microglia pretreated or not with 210 nM C16 and exposed or not to 20 μM Aβ42 in serum-free medium and treated with 200 pg/mL of IL-1β for 48 hours, treated or not with an autophagic flux inhibitor bafilomycin A1 (Baf) at 50 nM for 24 hours before cell lysis. Densities were quantified using GeneTools software. Data of each protein were reported to data of the corresponding β-actin. The results are expressed as arbitrary units (percentage of the control set at 100%) and are mean ± SEM from six independent experiments in duplicate. *** P <0.001 compared to control; §§§ P <0.001 compared to Aβ42; &&& P <0.001 compared to C16 by one-way ANOVA with a Newman-Keuls multiple comparison test. Baf, bafilomycin A1; mTOR, mammalian target of rapamycin.