Figure 4
Activation of mTOR signaling pathway by inflammatory stress in primary tri-cultures. Representative immunoblots show the immunoreactivity of (A) mTOR, PS2448-mTOR, (B) p70S6K, PT389-p70S6K, and β-actin from cell lysates of primary tri-cultures exposed to 20 μM Aβ42 or 100 ng/mL LPS pretreated or not with 210 nM C16 in serum-free medium for 48 hours, treated or not with an autophagic flux inhibitor bafilomycin A1 (Baf) at 50 nM 24 hours before cell lysis. Densities were quantified using GeneTools software. Data of each protein were reported to data of the corresponding β-actin. The results are expressed as arbitrary units (percentage of the control set at 100%) and are mean ± SEM from six independent experiments in duplicate. *** P <0.001 compared to control; ††† P <0.001 compared to LPS; ### P <0.001 compared to Baf; ‡‡‡ P <0.001 compared to LPS with Baf; & P <0.05 compared to C16 by one-way ANOVA with a Newman-Keuls multiple comparison test. Baf, bafilomycin A1; LPS, lipopolysaccharide, mTOR, mammalian target of rapamycin.