Figure 8From: Involvement of interleukin-1β in the autophagic process of microglia: relevance to Alzheimer’s disease Cytokine levels induced by inflammatory stress in microglia. Cytokine (IL1-β, TNF-α, and IL-6) levels in cell lysates (A, B, C, respectively) and in culture medium (D, E, F, respectively) of primary microglia pre-treated or not with 210 nM C16, exposed or not to 20 μM Aβ42, and treated with 200 pg/mL of IL-1β in serum-free medium were analyzed by the 3-plex Luminex xMAP assay containing a mixture of specific beads for each cytokine as described in the Methods section. Cytokine levels in cell lysates and culture medium are expressed in pg/mg protein and pg/mL, respectively. Results are mean ± SEM for six experiments in duplicate. * P <0.05, ** P <0.01, *** P <0.001 compared to respective control; † P <0.05, †† P <0.01, ††† P <0.01 compared to IL-1β; ϵ P <0.05, ϵϵ P <0.01, ϵϵϵ P <0.001 compared to Aβ42 with IL-1β by one-way ANOVA with a Newman–Keuls multiple comparison test.Back to article page