Figure 3From: Inflammasome induction in Rasmussen’s encephalitis: cortical and associated white matter pathogenesis Inflammasome expression in primary human neural cells. (A) Immune gene transcript levels in primary human microglia, astrocytes, and neurons showing that microglia express all inflammasome-associated genes examined unlike astrocytes or neurons. (B) Expression of inflammasome components and substrates in primary microglia ± TNF-α (50 ng/mL). (C) IL-1β expression in LPS (25 ng/mL) primed microglia co-stimulated with ATP (500 M) or MDP (500 ng/mL) ± caspase inhibitor zVAD-fmk (100 M). (D) IL-1β release from LPS (25 ng/mL) primed microglia (but not astrocytes) co-stimulated with ATP (500 μM) or MDP (500 ng/mL). Student’s t-test, *P <0.05.Back to article page