Figure 2

GluR2 mRNA and protein expression in activated mixed glial cultures. Quantitative real-time PCR (A-D) and western blotting (E) were performed to detect the AMPA subunit, GluR2, expressed in mixed glial cultures in the absence and presence of TNF-α (A, C) or LPS (B, D). Data are presented as fold change in gene expression relative to time-matched controls and standardised to 18 s or to all other GluR subunits. Protein expression is relative to β-actin + SEM of four independent experiments. * P <0.05.