Figure 6

NR1 mRNA and protein expression in activated mixed glial cultures. Quantitative real-time PCR (A and B) and western blotting (C) were performed to detect the NMDA subunit NR1 and AMPA subunit GluR2 expressed in mixed glial cultures in the absence and presence of TNF-α or LPS. Data are presented as fold change in gene expression relative to time-matched controls and standardised to 18 s + SEM of four independent experiments. * P <0.05.