Figure 2

HGF reduces inflammatory cytokine and cytolytic enzyme production by activated CD8 ⁺ T cells. Pmel-1 splenocytes were cultured for 5 days in IL-2 alone or in combination with HGF, following antigen-specific stimulation. (a and b) Intracellular cytokine staining of day 5-cultured CD8⁺ T cells showed that addition of HGF not only decreased (a) the number of CD8⁺ T cells producing IFN-γ, granzyme B, and perforin but also decreased (b) the amount of IFN-γ, granzyme B, and perforin production on a per cell basis, as indicated by comparative geometric mean of fluorescence (GMEAN) ± SEM of three independent experiments. Final GMEAN values are the result of the ratio between the GMEAN obtained with the experimental antibody and the isotype control. Representative contour plots (a) and histograms (b) are shown. *P <0.05 by Student’s t-test. All data were obtained from three independent experiments with similar results.