Figure 3

NOX2 expression increases significantly ipsilateral to the injury site. Immunofluorescence detection for NOX2 was performed on tissue sections from naïve/sham animals and animals at 24 hours, 7 days, and 28 days after moderate brain injury. Protein expression was measured from the midline to the edge of the injury site by densitometry analysis using stain intensity threshold. NOX2 staining increased by 24 hours post-injury, and was reduced at 7 and 28 days post-injury, although it remained significantly greater than naïve/sham at 28 days. Points represent individual measurements; line at mean and SD. *P <0.05 in comparison to naïve/sham.