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Figure 6 | Journal of Neuroinflammation

Figure 6

From: Erythropoietin improves motor and cognitive deficit, axonal pathology, and neuroinflammation in a combined model of diffuse traumatic brain injury and hypoxia, in association with upregulation of the erythropoietin receptor

Figure 6

Loss of dendrites is regionally variable in traumatic axonal injury (TAI) and traumatic axonal injury with hypoxia (TAI + Hx) rats, and is largely rescued by erythropoietin (EPO). (A) Representative photomicrograph of dense MAP2 immunoreactivity observed in the lateral septal nucleus of a sham-treated rat. (B) 1 d after TAI, there was a diffuse loss of MAP2 staining, although the cytoarchitecture remained similar to sham. (C) EPO treatment improved the appearance of MAP2 reactivity in the lateral septal nu. (D) Striking changes in MAP2 staining were observed in TAI + Hx rats, in which changes both in the intensity of MAP2 staining with regions completely devoid of MAP2 immunoreactivity. (E) EPO markedly rescued dendritic loss in TAI + Hx + EPO rats. Scale bar = 100 μm. (F) Densitometric analysis of TAI rats revealed a decrease in MAP2 labeling at 1 d compared to sham, and was significantly improved by EPO treatment. (G) TAI + Hx rats had a more substantial loss of MAP2 1 d postinjury, and EPO treatment significantly restored MAP2 density to sham levels. With this protective effect maintained at 7 and 14 d. Data shown as mean ± SEM, n = 6 per group per time point. *P <0.05, 2-way ANOVA with post-hoc Bonferroni test.

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Journal of Neuroinflammation

ISSN: 1742-2094