Figure 2
Effect of LPS on the intracellular levels of pro- and mature BDNF in the presence of a selective A 2A R antagonist in murine N9 microglial cells. The A2AR antagonist SCH58261 (50 nM) prevented the LPS (100 ng/mL)-induced modification of mature BDNF levels selectively at 6 hours (B). In contrast, the A2AR blockade failed to affect the levels of pro- and mature BDNF at 3 hours (A) or 12 hours (C) of LPS exposure. Note that A2AR only affected BDNF levels when they were challenged with LPS. Results are expressed as mean ± SEM of n (as indicated in each bar) independent experiments (*** P < 0.001, compared with non-treated cells; ·· P < 0.01, compared with LPS-treated cells, using the Newman–Keuls multiple comparison test) and 100% represents the pro- and mBDNF in cells that were not exposed to LPS. (D) Representative blot of the modulation by SCH58261 of intracellular mature BDNF in cells challenged for 6 hours with LPS. We verified (not shown) that none of the vehicles of the tested drugs (water or dimethyl sulfoxide) modified BDNF levels. A2AR, A2A receptor; BDNF, brain-derived neurotrophic factor; LPS, lipopolysaccharide; mBDNF, mature protein BDNF; SEM, standard error of the mean.