Inhibitory effects of HPB242 on
accumulation of Aβ
in the brain of Tg2576 mice. Aβ
accumulation in the brains of Tg2576 mice was determined by
immunohistochemical analysis using
Aβ1-42-specific antibody (A). The sections of Tg2576 mouse
brains were incubated with anti-Aβ1-42
primary antibody, and biotinylated secondary antibody.
Immunoperoxidase staining of brains of Tg2576 and treated-Tg2576
mice shown (brown color). Aβ1-42 level
was measured in mouse brains by ELISA as described in Materials
and Methods (B). The value is
mean ± standard error of the mean (SEM) (n = 8 mice). The
activity of β-secretase was investigated using assay kit as
described (C). Values measured
from each group of mice were calibrated by the amount of protein
and expressed as mean ± SEM (n = 8 mice). The expression of APP
and BACE1 were detected by western blotting using specific
antibodies in the mouse brain. Each blot is representative of
three experiments (D).
*Significantly different from non-treated Tg2576 mice (P < 0.05).