Figure 6

Effects of HPB242 on signal transducer and activator of transcription (STAT)1 and STAT3 activation in the Tg2576 mice. Gel electromobility shift assay (EMSA) analysis of STAT1 and STAT3 DNA binding activity in nuclear extracts from the brains (cortex and hippocampus) of HPB242-treated and non-treated Tg2576 mice(A). The retarded bands are indicated by an arrow. Representative results were obtained from at least three different sets of experiment (n = 8). The Tg2576 mouse brain extracts were prepared, and phospho-STAT1 and phospho-STAT3 level were detected by western blot (B). Each blot was representative of three experiments (n = 8).