Effect of 5-Aza treatment. Cells were treated with 5-Aza (0.01, and 1 μM) for 3 days and then subjected to oxygen glucose deprivation and reperfusion (OGD+R). RNA extracted from the cells was used for reverse transcription polymerase chain reaction (RT-PCR). Metallothionein (MT) induction was potentiated by 5-Aza treatment in a dose-dependent manner. Values represent the mean ± S.E.M. of three different experiments. *P <0.05 compared to 0 uM of 5 Aza; #P <0.05 compared to the control group.