Effect of ischemia or hypothermia on methylation profiles of the 5
-regulatory region of the metallothionein 1 (MT1) promoter. (A) Partial sequences of the 5′-regulatory region of the mouse MT1 is based on Gene Bank nucleotide sequence [GenBank: J00605]. Twenty-nine CpG sites are marked in red. Some transcriptionally active binding elements such as metal response elements (MRE), signal transducer and activator of transcription (STAT), and antioxidant response elements (ARE) are shown in blue. (B) Methylation profiles of the normothermia control and normothermia oxygen glucose deprivation and reperfusion (OGD+R) were compared. (C) Methylation profiles of the hypothermia control and hypothermia OGD+R were compared. Bisulfite pyrosequencing analysis was performed for 29 CpG sites near the transcription initiation site of the 5′-regulatory region. Methylated status of the CpG sites is expressed as an open circle (○) for unmethylated and a black circle (●) for methylated.