Figure 8
From: Hypothermia enhances induction of protective protein metallothionein under ischemia
Effect of ischemia or hypothermia on methylation regulating factors (A). We measured the mRNA levels of methylation related proteins such as DNA methyltransferases (DNMTs) 1, 2, 3, and 4 using real-time PCR. RNA was harvested from cells exposed to 4 hours of oxygen glucose deprivation (OGD) and to reperfusion for 3 to 24 hours. (B) The levels of methyl-CpG binding domain proteins (MBDs) 1, 2, 3, and 4 along with methyl-CpG binding protein-2 (MECP-2) were measured using the same samples and analysis tools as in 6A. Values represent the mean ± S.E.M. of three different experiments.