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Figure 11 | Journal of Neuroinflammation

Figure 11

From: Toll-like receptor 4 mediates microglial activation and production of inflammatory mediators in neonatal rat brain following hypoxia: role of TLR4 in hypoxic microglia

Figure 11

Toll-like receptor 4 (TLR4) inhibition suppressed the increase in TNF-α and IL-1β immunoexpression on microglia in the corpus callosum and cerebellum in neonatal rats after hypoxic treatment. Confocal images showing the expression of TNF-α and IL-1β in lectin-labeled (green) microglia in the corpus callosum (A) and cerebellum (B) of control, hypoxia and hypoxia + CLI-095 rats at 3 days after the hypoxic exposure. (A) Increase in TNF-α and IL-1β expression in microglia of the corpus callosum was evident in hypoxic rats. In hypoxia + CLI-095 rats, both increase in TNF-α and IL-1β was inhibited compared with the hypoxia rats. (B) TNF-α was undetected in the cerebellar microglia of normal rats (b) but its immunoexpression was intensely induced in hypoxic rats, while in hypoxia + CLI-095 rats the immunofluorescence intensity was attenuated. IL-1β immunofluorescence intensity was enhanced after hypoxia but the increase was inhibited in hypoxia + CLI-095 rats. Scale bars in A and B = 20 μm.

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