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Figure 2 | Journal of Neuroinflammation

Figure 2

From: Toll-like receptor 4 mediates microglial activation and production of inflammatory mediators in neonatal rat brain following hypoxia: role of TLR4 in hypoxic microglia

Figure 2

Toll-like receptor 4 (TLR4) expression was increased in primary cultured microglia following hypoxia. (A) Confocal images showing the expression of TLR4 (Ab, Ae; red) in primary cultured microglia labeled with lectin (Aa, Ad; green) in both control and hypoxia for 24 h. TLR4 immunoflurosence intensity is markedly enhanced after hypoxia exposure (Af) in comparison with the control (Ac). Nuclei are stained with DAPI (blue). (B) TLR4 was neutralized with its antibody in control and hypoxia conditions. In control microglia, microglia + TLR4 Ab, microglia + hypoxia and microglia + hypoxia + TLR4 Ab, there is no noticeable difference in cell morphology between different groups under the phase-contrast microscope. Scale bars = 20 μm (A) and 100 μm (B).

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Journal of Neuroinflammation

ISSN: 1742-2094