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Figure 4 | Journal of Neuroinflammation

Figure 4

From: Toll-like receptor 4 mediates microglial activation and production of inflammatory mediators in neonatal rat brain following hypoxia: role of TLR4 in hypoxic microglia

Figure 4

Hypoxia-inducible factor-1 alpha (HIF-1α) expression in hypoxic BV-2 cells. (A) The external cell morphology appears relatively unchanged in BV-2 cells exposed to hypoxia for 8 h when compared with the control. (B) Bar graph shows the viability of BV-2 cells after hypoxia compared to control. Note that the viability of cells in the two groups is comparable. (C) Reverse transcription (RT)-PCR analysis of HIF-1α mRNA expression in BV-2 cells exposed to hypoxia for 2, 4, and 6 h and control (c). Note that there is no significant difference between different groups. (D) Western blot analysis of HIF-1α protein expression in different groups. Specific band of HIF-1α (120 kDa) and β-actin (43 kDa) is shown in the upper panel of D. Bar graphs in the lower panel of D shows significant changes in the optical density following 2 and 4 h of hypoxic exposure (normalized with β-actin, shown as fold change of control), notably after exposure to hypoxia for 4 h. (E) Confocal images showing the expression of HIF-1α in BV-2 cells and those exposed to hypoxia for 4 h. Weak HIF-1α expression is detected in the control BV-2 cells (Eb, Ec). The immunofluorescence intensity is enhanced markedly after hypoxic exposure (Ee, Ef). Significant differences in protein levels between hypoxic and control BV-2 cells are indicated as *P <0.05 and **P <0.01. The values represent the mean ± SD in triplicate. Scale bars = 100 μm (A) and 20 μm (E).

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