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Figure 5 | Journal of Neuroinflammation

Figure 5

From: Toll-like receptor 4 mediates microglial activation and production of inflammatory mediators in neonatal rat brain following hypoxia: role of TLR4 in hypoxic microglia

Figure 5

Toll-like receptor 4 (TLR4) expression was increased in BV-2 cells following hypoxia. (A) Western blotting of TLR4 protein expression in BV-2 cells exposed to hypoxia for 2, 4, 6, 8 and 12 h and control (c). The left panel shows specific bands of TLR4 (95 kDa) and β-actin (43 kDa). The right panel is a bar graph showing significant changes in the optical density following hypoxic exposure (normalized with β-actin, shown as fold change of control). Note significant increase in TLR4 expression after hypoxic treatment of varying durations in BV-2 cells, especially at 8 h. (B) Confocal images showing TLR4 expression in control BV-2 cells and those exposed to hypoxia for 8 h. Weak TLR4 expression is detected in the control BV-2 cells (Bb, Bc), with enhanced immunofluorescence intensity after 8 h of hypoxic exposure (Be, Bf). Significant differences between control and hypoxic BV-2 cells are expressed as *P <0.05 and **P <0.01. The values represent the mean ± SD in triplicate. Scale bar in B = 20 μm.

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Journal of Neuroinflammation

ISSN: 1742-2094