C5L2 and CD88 expression is upregulated in AD cases. A. Control and AD extracts from hippocampus subjected to SDS polyacrylamide gel and transferred to PVDF membranes were probed sequentially with anti human C5L2 antibody (N1-23 epitope) upper lane, anti human CD88 (Abcam, C terminal epitope) (middle lane) and anti ß actin (lower lane). B. Western blot of hippocampus lysates of AD case probed with C5L2 N1-23 antibody with (left lane) and without (right lane) preadsorption of the antibody with the N1-23 peptide. The 37 k Mr band is eliminated by preadsorption. C. Western blot of hippocampus homogenates of control and AD cases (from the Rush Religious Order Study) using Abcam polyclonal antibody against aa248-311of C5L2 and ß actin antibody for further densitometric quantification. D. Densitometric quantification of C5L2 immunoblots. Bars show average of ratio of C5L2/ßActin +/− SE from Figure
3A which are AD (n = 5) 0.31 +/−0.05 and C (n = 4) 0.12+/− 0.03, P <0.01 (left) or from Figure
3C and data not shown AD (n = 12) 1.05+/−0.05 and C (n = 12) 0.72+/− 0.03, P <0.01 (right). AD, Alzheimer’s disease; C, control; n, number; PVDF, polyvinylidene difluoride.