Figure 5

Effects of RA on phosphor- NF- κB, phosphor- IκB- α and HMGB1 in cerebral I/ R rats by western blots analysis 24 h after I/ R. Diabetic rats were subjected to middle cerebral artery occlusion (MCAO) and reperfusion (I/R) for 23 h. Total protein extracts were prepared and assayed for phosphor-NF-κB, phosphor-IκB-α and HMGB1 by Western blot analysis, and blots were normalized to PCNA expression. RA indicates rosmarinic acid. Results are expressed as fold increase over sham group, n = 5. ^* P <0.01 vs. Vehicle-treated group. ^# P <0.01 vs. Diabetic group. Significance was determined by one-way analysis of ANOVA followed by Dunnett’s test.