Differential effects of colonic inflammation on PKC and ROK signaling in the bladder smooth muscle in mice lacking TRPV1 receptors. (A) Representative raw tracers of the detrusor contractile responses to protein kinase C (PKC) activator (phorbol-12,13-dibiturate (PDBU), 1 μM) in wild-type (WT) and transient receptor potential vanilloid 1 knockout (TRPV1−/−) mice. (B) Normalized contractile response of isolated detrusor smooth muscle (DSM) strips in response to PDBU in WT and TRPV1 KO mice. (C) DSM relaxation response upon application of Rho-kinase (ROK) inhibitor Y27632 (20 μM) after PDBU-induced contractions. The maximal amplitude of contraction induced by PDBU was taken as 100%. Star symbols mark the statistical significance between control and treatment groups whereas pound symbols reflect significant difference between WT and KO mice. (D) Normalized contractile response of detrusor muscle contractions upon stimulation with high potassium chloride (KCl, 125 mM) after 30 min incubation of muscle strips with ROK inhibitor Y27632 (N = 4, n = 8 in all groups).