Figure 10

Cytokines and chemokines released during polymorphonuclear neutrophils (PMN) transmigration (TM). The supernatants of transwell filters were analyzed after PMN TM experiments for different cytokines/chemokines (pg/ml) with cytokine bead array (CBA) after stimulation of human choroid plexus papilloma cells (HIBCPP) with strain α14, strain MC58, strain MC58ΔsiaD or TNFα. The cyotokines IL-6, macrophage inflammatory protein (MIP)-1α, MIP-1β, IL-1β were strongly secreted after bacterial infection of HIBCPP and PMN TM (A-D), whereas GRO (CXCL1,2,3), monocyte chemoattractant protein (MCP)-1, Interferon gamma-induced protein-10 (IP-10) and RANTES (regulated and normal T cell expressed and secreted) were already secreted after bacterial stimulation of HIBCPP alone (E-H). TNFα and IL-8 were also released during PMN transmigration and after infection of HIBCPP (I-J). Data shown are means ± SD of two independent experiments and two filters per condition analyzed in duplicate. ^* P <0.05, ^** P <0.01 compared to stimulated HIBCPP with and without PMNs. ^# P <0.05 compared to TNFα-stimulated cells.