Cytokines and chemokines released during monocyte transmigration. The supernatants of transwell filters were analyzed after monocyte TM experiments for different cytokines/chemokines (pg/ml) with cytokine bead array (CBA) after stimulation of human choroid plexus papilloma cells (HIBCPP) with strain α14, strain MC58, strain MC58ΔsiaD or TNFα. IL10, IL1α, IL1β, IL-6 and TNFα display a group of cytokines mainly secreted after monocytes have migrated through infected HIBCPPs (A-E). GRO (CXCL1,2,3,) and IL-8 were already secreted by HIBCPP when stimulated with bacteria alone (F-G). Macrophage inflammatory protein (MIP)-1α and MIP-1β were released after bacterial stimulation of monocytes (H-I). Data shown are mean ± SD of two independent experiments and two filters per condition analyzed in duplicate. *
P <0.05; **
P <0.01; ***
P <0.001 compared to stimulated HIBCPP with and without monocytes. #
P <0.05; ##
P <0.01; ###
P <0.001 compared to TNFα-stimulated cells.