Figure 12

Signal regulatory protein (SIRP)α expression on transmigrated monocytes and polymorphonuclear neutrophils (PMNs). Lysates of monocytes and PMNs from the transmigration experiment involving stimulated (MC58, TNFα or control) human choroid plexus papilloma cells (HIBCPP) were used for western blotting. SIRPα protein levels of the neuronal (80 to 85 kDa) and its deglycosylated form (50 to 60 kDa) were determined. The figure shows a representative example of five independent experiments that all gave similar results.