Figure 6

Actin cytoskeleton morphology in HIBCPP after immune cell transmigration. En face Apotome® microscopy images, showing human choroid plexus papilloma cells (HIBCPPs) stained for the tight junction (TJ) protein zonula occludens (ZO)-1 (red), the actin (phalloidin, yellow) and cell nuclei (4‘-6-diamidino-2-phenylindole dihydrochloride (DAPI), blue), polymorphonuclear neutrophils (PMNs) stained with a neutrophil marker (CD66b-fluorescein isothiocyanate (FITC), green) and monocytes labeled with CD16-FITC (green). Shown is actin cytoskeleton morphology of HIBCPP after PMN (A) and monocyte (B) transmigration under different simulation conditions (α14, MC58, MC58ΔsiaD, TNFα and control conditions). Actin cytoskeleton analysis showed no major differences in actin morphology between control and stimulated HIBCPP cells as well as in PMN or monocyte experiments. Of note, the visualized monocytes are obsverved very rarely especially after bacterial stimulation. The figures show representative examples of three independent experiments that all gave similar results. Scale bar as indicated.