Figure 1From: In vivo temporal and spatial profile of leukocyte adhesion and migration after experimental traumatic brain injury in mice Experimental design. (A) Schematic illustration of the imaging window and controlled cortical impact (CCI)-induced contusion and penumbra sites. (B) Timeline of the experiments for monitoring the cerebral microcirculation, including baseline recording. (C) For observations up to 14Â h post-trauma, the mice were allowed to recover in their respective cages until the preparation for intravital microscopy (IVM). (D) Following an injection of either anti-CD18 antibodies or control IgG, the animals were subjected to CCI; 13Â h thereafter, the mice were prepared for IVM. All IVM experiments ended with perfusion-fixation with paraformaldehyde and the subsequent removal of the brain. (E) The animals received either control IgG or anti-CD18 antibodies and were then subjected to CCI; 15Â min or 24Â h later, their brains were removed for histological assessment.Back to article page