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Figure 1 | Journal of Neuroinflammation

Figure 1

From: TNF-α promotes cerebral pericyte remodeling in vitro, via a switch from α1 to α2 integrins

Figure 1

Extracellular matrix (ECM) substrates differentially regulate cerebral pericyte behavior. (A) Phase pictures showing pericyte adhesion and morphology on collagen I, collagen IV, fibronectin, HSPG, and laminin-1 after 1 and 4 hours. Scale bar = 100 μm. Note that pericytes attached well to fibronectin and the collagens but very poorly to HSPG. (B) Time course of pericyte adhesion to different ECM substrates over 8 hours. Adhesion assays were performed as described in the text, and all points represent the mean ± SEM of four experiments. Note that after 1 hour, the number of adherent pericytes was >90% on fibronectin, approximately 40% on collagens, approximately 10% on laminin-1, but less than 1% on HSPG. * P<0.01, ** P<0.001. (C) Influence of ECM substrates on pericyte proliferation. Proliferation assays were performed as described in the text, and all points represent the mean ± SEM of four experiments. Note that fibronectin strongly promoted (** P<0.01), whereas laminin-1 (* P<0.05) and HSPG (** P<0.01) inhibited pericyte proliferation. (D) Influence of ECM substrates on pericyte migration. Migration assays were performed as described in the text, and all points represent the mean ± SEM of four experiments. Note that pericyte migration was promoted most strongly by collagen I (*** P<0.001) and to a lesser degree by fibronectin (** P<0.01) and laminin-1 (* P<0.02).

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