Figure 3
From: TNF-α promotes cerebral pericyte remodeling in vitro, via a switch from α1 to α2 integrins
Tumor necrosis factor (TNF)-α strongly promotes a pericyte remodeling phenotype. (A) The influence of TNF-α on pericyte morphology. Scale bar = 50 μm. Note that TNF-α promoted a switch in pericyte morphology, from a well-spread, rhomboid phenotype into a predominantly bipolar, polarized morphology. (B) The influence of TNF-α on pericyte migratory capacity. Scale bar = 100 μm. Note that TNF-α enhanced pericyte migration, as illustrated by a rapid closing of the scratch defect. (C,D) Influence of cytokines on (C) pericyte migration and (D) proliferation. Assays were performed as described in the text, and all points represent the mean ± SEM of four experiments. Note that pericyte migration and proliferation were promoted most strongly by TNF-α * P<0.05, ** P<0.01).