Figure 2

Expression of markers on M0, M1 and M2 macrophages stimulated in vitro. Macrophages were polarized to M1 and M2 macrophages (see materials and methods for details) and the expression of markers was analyzed by flow cytometry. In all figures the black bold line represents M1 macrophages (A). CD40 and CD64 are both shifted in mean fluorescent intensity; however, only CD40 is significantly upregulated on M1 macrophages compared to M0 macrophages. The M2 macrophage population is depicted by a grey line. Mannose receptor (MR) expression is upregulated on the M2 macrophages, whereas no differences in CD163 expression were observed comparing the different subsets. The means ± SEM were calculated from three independent experiments performed in duplicate and *P <0.05 calculated for mean fluorescent intensity (MFI) was considered significant (B).