Table 2 Antibodies used in fluorescence-activated cell sorting (FACS) analysis and immunohistochemistry (IHC)
From: Macrophages in inflammatory multiple sclerosis lesions have an intermediate activation status
Antigen | Species | Dilution (FACS) | Dilution (IHC) | Double stain (IHC) | Manufacturer |
|---|---|---|---|---|---|
CD68 (KP1) | mouse | 1:100 | 1:1000 | 1:1000 | Dako |
CD40 (Clone MCA1590) | mouse | 1:50 | 1:1500 | 1:750 | Serotec |
CD86 (MCA1118) | mouse | 1:50 | 1:400 | - | Serotec |
CD64 (555525) | mouse | 1:50 | 1:250 | - | Serotec |
CD32 (MCA1075) | mouse | 1:500 | 1:2000 | - | Serotec |
CD206 (Clone 19.2) | mouse | 1:100 | 1:400 | 1:250 (bio 1:50) | BD Pharmingen |
CD163 (ED1) | mouse | 1:50 | 1:1000 | - | Serotec |
HLA-DR (LN3) | mouse | - | 1:1000 | 1:1000 | Dako |