Figure 2

Assessment of human microglial IGF1 and IGF2 mRNA expression by Q-PCR. Microglia cultures were treated with IFNγ (10 ng/ml), IL-13 (10 ng/ml), IL-4 (10 ng/ml), LPS (100 ng/ml), poly(I:C) (10 μg/ml), or medium alone for 6 h and Q-PCR was performed for IGF1, IGF2, IGF2R, and TNFα. Fold changes over control (medium alone) were calculated in each case and values from multiple microglial cases were pooled and statistical significance analyzed using one sample t test (GraphPad Prism 5) * P < 0.05, ** P < 0.01, *** P < 0.001. IGF1 mRNA was suppressed by IFNγ, LPS, or poly(I:C) (A), IGF2 mRNA increased by LPS (B), IGF2R mRNA increased by IFNγ (C), and TNFα mRNA increased by IFNγ, LPS or poly(I:C) (see broken y axis) and suppressed by IL-4 or IL-13 (D).