Figure 2

Microglia are pleomorphic after 60 min of focal ischemia. A Representative confocal maximum intensity projection images of iba-1 positive microglia and magnified skeletonized inset in brain regions (1 striatum; 2–4 cortex) after 60 min of focal ischemia. B Microglia process endpoints/cell are significantly increased in ipsilateral region 1 but decreased in regions 3 and 4 versus matching contralateral regions. C Microglia process length/cell was significantly decreased in ipsilateral region 4 versus contralateral hemisphere. Data were tested for statistical differences between ipsilateral vs. contralateral regions using Student’s t-test (n = 10, *p < 0.05, **p < 0.01 and ***p < 0.001). All data are mean ± SEM. Scale bar = 10 μm.