Figure 3From: A quantitative spatiotemporal analysis of microglia morphology during ischemic stroke and reperfusion Microglia are de-ramified in brain regions proximal to cerebral injury after ischemic stroke and 8 h of reperfusion. A Representative confocal maximum intensity projection images of iba-1 positive microglia and magnified skeletonized inset in brain regions (1 striatum; 2–4 cortex) after 60 min of focal ischemia and 8-h reperfusion. B The number of microglia process endpoints/cell was significantly reduced in ipsilateral regions 2 and 3 versus matching contralateral regions. C Microglia process length/cell was significantly reduced in ipsilateral regions 2 and 3 versus matching contralateral hemispheres. Data were tested for statistical differences between ipsilateral vs. contralateral regions using Student’s t-test (n = 7 *p < 0.05 and **p < 0.001). All data are mean ± SEM. Scale bar = 10 μm.Back to article page