Tissue iNOS expression is not altered during the first 24 h after cerebral ischemia and reperfusion.
A Brain tissue for Western blot analysis of iNOS expression was acquired in regions 1–3 (1 striatum; 2, 3 cortex) as illustrated in the TTC image of a typical area of injury after ischemic stroke and 24-h reperfusion. Brain regions were conserved across all time points in the ipsilateral and contralateral hemispheres. B iNOS expression, normalized to β actin, is not significantly changed relative to the contralateral hemisphere (100%) after 60 min of focal ischemia (n = 5), C nor after ischemic stroke and 8 h (n = 4) or D ischemic stroke and 24 h of reperfusion (n = 5). E iNOS expression was significantly increased in the cortical ipsilateral region proximal to the necrotic core after 60 min of ischemia and 72 h of reperfusion (n = 3). Data were tested for statistical differences in iNOS expression from contralateral (100%) using a one-sample t-test (*p < 0.05). All data are mean ± SEM.