Figure 8

CD11b expression is increased in regions of hyper-ramified but not de-ramified microglia after 60 min of focal cerebral ischemia. A Representative confocal maximum intensity projection images of CD11b expression in ipsilateral and contralateral brain regions (1 striatum; 2–4 cortex) after 60 min of focal ischemia. B Enlarged image of microglia (top panel, iba-1) and corresponding CD11b expression (bottom panel) from boxed region as depicted in (A). C Microglia CD11b expression was significantly increased in ipsilateral regions 1 and 2 versus matching contralateral hemispheres. Data were tested for statistical differences between ipsilateral vs. contralateral regions using Student’s t-test (n = 10, *p < 0.05). All data are mean ± SEM. Scale bar = 10 μm.