Mouse mid-brain derived SN4741 cells express GHS-R, GOAT, BIII-tubulin and TH. To determine whether SN4741 neurones were an appropriate cellular system to study ghrelin’s neuronal actions, we performed immunofluorescence (IF) to establish expression of key proteins. SN4741 cells were immunoreactive for βIII-tubulin (A), TH (B), GHS-R1a (C) and GOAT (D). No immunoreactivity was observed for the glial marker, GFAP (A). For all negative controls the primary antibodies were excluded from the staining procedure. In addition, specificity of the GOAT antibody has been confirmed using an antibody-specific blocking peptide (Phoenix peptide cat.no 032–12; personal communication, Dr T Wells). The images are representative of three independent experiments performed with three replicates for each antigen.