Figure 1

Central fluid percussion injury (cFPI) procedure. (A) To prepare for cFPI, a 3.0 mm diameter craniotomy was made in the midline between the bregma and lambda sutures, leaving the underlying dura intact; (B) Using dental cement, a cap filled with normal saline was sealed over the craniotomy; (C) Overview of the bregma levels where the different immunohistochemical analyses were prepared.