Figure 7
Overview of the distribution of microglial, neutrophil and T-cell staining at 1, 3 and 7 days after cFPI or sham-injury in mice.(A-C) The microglial staining was increased throughout the observation period in the cortex and in the subcortical white matter, especially at one day post-injury. Infiltrating neutrophils appeared at one day post-injury in the cortex and subcortical white matter. T-cells were observed in the cortex and in the subcortical white matter after one day post-injury but then the number of cells declined; (D) The inflammatory response after cFPI or sham-injury. The images show activated microglia/macrophages (MAC-2), neutrophils (GR-1) and T-cells (CD3) in the external capsule from post-injury day (PID) 1 to 7, examples of cells staining positive for the cell type marker are indicated with arrows. Inserts show the same area in the external capsule at a higher magnification; (E) Cell counts of microglia/macrophages, neutrophils and T-cells in the corpus callosum, external capsule and fimbria of hippocampus from 1, 3 and 7 days post-injury (presented as means ± SD). *Indicates a statistically significant difference compared to sham-injured controls (P<0.05).