Figure 2

Poly(ADP-ribose) polymerase 2 (PARP-2) deletion reduces spinal inflammation. (A-C) Peak experimental autoimmune encephalomyelitis (EAE) disease ((B) wild-type and (C) PARP-2 null) and sham control (A) spinal cords were subjected to hematoxylin and eosin staining. Wild-type EAE cords have substantial cellular infiltration to the parenchyma (B) while the sham control (A) and PARP-2 EAE cords (C) have minimal visible infiltration. Results are quantified in (D). Arrows point to perivascular foci of inflammatory infiltration. Scale bars are 200 μm (top row, (A-C)) and 25 μm (bottom row, (A-C)); bottom row in (A-C) represents magnification of top row framed areas. Values are mean ± SEM (n = 3 to 7). Results in (D) were analyzed using Kruskal-Wallis non-parametric analysis of variance (ANOVA) with Dunn’s multiple comparisons test. **P <0.01 compared to sham; *P <0.05 compared to wild-type EAE group; not significant = P >0.05.