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Figure 3 | Journal of Neuroinflammation

Figure 3

From: Poly(ADP-ribose) polymerase 2 contributes to neuroinflammation and neurological dysfunction in mouse experimental autoimmune encephalomyelitis

Figure 3

Poly(ADP-ribose) polymerase 2 (PARP-2) deletion reduces Th1 and Th17 T helper cell infiltration in experimental autoimmune encephalomyelitis (EAE) mice spinal cords. Sham control and peak EAE spinal cords from wild-type and PARP-2 null mice were subjected to coimmunofluorescence staining using antibodies for CD4 and T-bet (A-D) or CD4 and ROR-γT (E-H). Widespread CD4+ immunoreactivity (IR) was observed in wild-type EAE spinal sections (B), approximately 24% of which was also T-bet+. The inset shows a magnified image of the area directly to the right of the asterisk symbol (*) and illustrates cellular colocalization (yellow). (B) Representative image of reduced CD4+ and CD4+/T-bet+ infiltration in PARP-2 null EAE cords. (C,D) Quantifications of CD4+ and CD4+/T-bet+ cell counts and indicate a significant reduction of each with elimination of PARP-2. In (E), 12% of CD+ cells were also ROR-γT+. The inset in (E) shows a magnified image of the area directly to the left of the asterisk symbol (*). (F) Representative image of reduced CD4+ and CD4+/ROR-γT+ infiltration in PARP-2 null EAE cords. (G,H) Quantifications of CD4+ and CD4+/ROR-γT+ cell counts and indicate a significant reduction of each with elimination of PARP-2. Results in (C,D,G,H) are expressed as means ± SEM and were analyzed using one-way analysis of variance (ANOVA) with the Student Newman-Keuls multiple comparison test. **P <0.01 compared to sham; *P <0.05 compared to wild-type EAE; not significant = P >0.05. Scale bars are 25 μm.

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