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Table 1 Comparison of chemokine mRNA copy numbers in neurons, astrocytes and microglia derived from the rat cerebrum

From: Different actions of endothelin-1 on chemokine production in rat cultured astrocytes: reduction of CX3CL1/fractalkine and an increase in CCL2/MCP-1 and CXCL1/CINC-1

mRNA copy number

(× 10 3 /μg total RNA)

 

Neuron

Astrocyte

Microglia

CCL2/MCP-1

189.9 ± 9.8

3050.8 ± 521.5

148.9 ± 31.6

CCL5/RANTES

10.3 ± 0.7

52.5 ± 18.7

4.3 ± 2.2

CXCL1/CINC-1

22.0 ± 2.2

1410.0 ± 266.9

34.4 ± 18.1

CXCL12/SDF-1α

92.1 ± 4.0

255.4 ± 45.7

29.1 ± 13.6

CX3CL1/fractalkine

1,118.1 ± 108.4

1,721.0 ± 303.5

23.4 ± 6.6

G3PDH

40,081.9 ± 7,335.2

46,575.5 ± 6,274.4

48,586.5 ± 5,733.6

  1. Cultured neurons, astrocytes and microglia were prepared from the cerebra of Wistar rats and total RNA was extracted. The copy numbers of chemokine mRNA were determined by quantitative RT-PCR. The copy numbers of G3PDH mRNA in the same samples were also determined as an internal standard. Data are the mean ± SEM of 6 to 10 different preparations presented as ×103 copy numbers/μg total RNA. G3PDH, glyceraldehyde-3-phosphate dehydrogenase; SEM, standard error of the mean.
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Journal of Neuroinflammation

ISSN: 1742-2094