Immunofluorescence staining for interleukin (IL)-6. (A-H) Immunofluorescence staining for IL-6 in sections of dorsal root ganglia (DRG) removed from different groups of rats. Sections of (A) lumbar (L-DRGn) and (B) cervical (C-DRGn) DRG of naive rats. (C,D) Representative sections of L4 DRG from ipsilateral (L-DRGi) and contralateral (L-DRGc) sides of rats 3 days after unilateral chronic constriction injury (CCI) of the sciatic nerve (CCI 3D). (E,F) Cervical DRG from the ipsilateral (C-DRGi) and contralateral (C-DRGc) sides of the same rats (G,H) Ipsilateral cervical (C-DRGi) and lumbar (L-DRGi) DRG 3 days after sham operation. Large neurons (thick arrows), medium and small neurons (thin arrows), satellite glial cells (arrowheads). The sections were treated with rabbit polyclonal anti-IL-6 antibody and tetramethyl rhodamine isothiocyanate (TRITC)-conjugated donkey anti-rabbit secondary antibody under identical conditions. Scale bars = 60 μm.