Figure 4

Decreased basal Egr-1 protein levels in APP mice hippocampus are partially restored in the dentate gyrus after 10 weeks of SSR240612 delivery. (A) Immunohistochemistry in the dorsal hippocampus revealed a drastic reduction in Egr-1 staining intensity (total gray value) in the CA1-CA2 fields (B), and in the number of Egr-1-positive nuclei in dentate gyrus DG, (C) of APP mice. SSR240612 treatment had no effect on Egr-1 expression in CA1 (B). (C) In contrast, following 10 weeks of delivery, SSR240612 partially countered Egr-1 reduction in the DG of APP mice. Quantified areas are delineated in A (arrows in WT; dashed boxes in APP) and magnified in B and C. See insets for higher magnifications. Scale bar: 300 μm. *P <0.05; ††P <0.01; †††P <0.001 (†: vs. WT; *: non-treated APP vs. treated APP). Two-way ANOVA followed by Newman-Keuls post hoc test. N = 5/group. APP, amyloid precursor protein; Egr-1, early growth response protein 1; WT, wild-type.