Figure 5

SSR240612 reduced soluble Aβ _1–42 in hemibrains and Aβ plaque load in cortex and hippocampus. (A,B) Levels of soluble Aβ_1–42 were significantly reduced in the brain of APP mice after 5 (A) and 10 weeks (B) of B₁R blockade, as observed by ELISA. (C,D) Following SSR240612 treatment, thioflavin-S staining (C) and 6E10 immunohistochemistry (D) revealed a significant reduction in the surface area occupied by mature and diffuse Aβ plaques (plaque load) in both cortex and hippocampus (including the dentate gyrus). Error bars represent SEM. *P <0.05; **P <0.01, Student’s t test. N = 4 to 6/group. Aβ, amyloid-beta; APP, amyloid precursor protein; B₁R, bradykinin receptor 1.